Article
Production of CFTR-null and CFTR-DeltaF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer.
The Journal of clinical investigation - 1 Apr 2008
Rogers Christopher S, Hao Yanhong, Rokhlina Tatiana, Samuel Melissa, Stoltz David A, Li Yuhong, Petroff Elena, Vermeer Daniel W, Kabel Amanda C, Yan Ziying, Spate Lee, Wax David, Murphy Clifton N, Rieke August, Whitworth Kristin, Linville Michael L, Korte Scott W, Engelhardt John F, Welsh Michael J, Prather Randall S
Abstract excerpt
Progress toward understanding the pathogenesis of cystic fibrosis (CF) and developing effective therapies has been hampered by lack of a relevant animal model. CF mice fail to develop the lung and pancreatic disease that cause most of the morbidity and mortality in patients with CF. Pigs may be better animals than mice in which to model human genetic diseases because their anatomy, biochemistry, physiology, size,...
Topics
- Alleles
- Animals
- Animals, Genetically Modified
- Cystic Fibrosis Transmembrane Conductance Regulator
- Dependovirus
- Fibroblasts
- Gene Expression Regulation
- Gene Targeting