Article
Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase.
The Journal of biological chemistry - 15 Jan 1992
Beltrán C, Kopecky J, Pan Y C, Nelson H, Nelson N
Abstract excerpt
A DNA fragment containing the gene encoding subunit C of vaculor H(+)-ATPase (V-ATPase) was cloned from a yeast library. The predicted amino acid sequence indicated that the C subunit consists of 373 amino acids with a calculated molecular mass of 42,287 Da. The protein from yeast is 37% identica...
Topics
- Amino Acid Sequence
- Blotting, Southern
- Catalysis
- Cloning, Molecular
- DNA, Fungal
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Phenotype
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- Sequence Alignment
- Vacuoles