Article
A bovine cDNA and a yeast gene (VMA8) encoding the subunit D of the vacuolar H(+)-ATPase.
Proceedings of the National Academy of Sciences of the United States of America - 17 Jan 1995
Nelson H, Mandiyan S, Nelson N
Abstract excerpt
Subunit D of vacuolar H(+)-ATPase (V-ATPase) from bovine chromaffin granules was subjected to partial proteolysis and amino acid sequencing. A cDNA encoding this subunit was isolated and sequenced. The predicted open reading frame encodes a protein of 247 amino acids with a calculated molecular weight of 28,336. Northern blot analysis revealed an mRNA distribution with higher transcript amounts in tissues that...
Topics
- Adrenal Medulla
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cattle
- Chromaffin Granules
- DNA, Complementary
- Gene Library
- Genes, Fungal
- Molecular Sequence Data
