Article
A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1.
Molecular systems biology - 1 Jan 2008
de Berardinis Véronique, Vallenet David, Castelli Vanina, Besnard Marielle, Pinet Agnès, Cruaud Corinne, Samair Sumitta, Lechaplais Christophe, Gyapay Gabor, Richez Céline, Durot Maxime, Kreimeyer Annett, Le Fèvre François, Schächter Vincent, Pezo Valérie, Döring Volker, Scarpelli Claude, Médigue Claudine, Cohen Georges N, Marlière Philippe, Salanoubat Marcel, Weissenbach Jean
Abstract excerpt
We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection...
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