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Article

Histidine-alpha143 assists 1,2-hydroxyl group migration and protects radical intermediates in coenzyme B12-dependent diol dehydratase.

Biochemistry - 11 Mar 2008

Kinoshita Koichiro, Kawata Masahiro, Ogura Ken-ichi, Yamasaki Ai, Watanabe Takeshi, Komoto Noriaki, Hieda Naoki, Yamanishi Mamoru, Tobimatsu Takamasa, Toraya Tetsuo

Abstract excerpt

Diol dehydratase of Klebsiella oxytoca contains an essential histidine residue. Its X-ray structure revealed that the migrating hydroxyl group on C2 of substrate is hydrogen-bonded to Hisalpha143. Mutant enzymes in which Hisalpha143 was mutated to another amino acid residue were expressed in Escherichia coli, purified, and examined for enzymatic activity. The Halpha143Q mutant was 34% as active as the wild-type...

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PMID: 18260646
DOI: 10.1021/bi7018095

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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