Article
Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols.
The FEBS journal - 1 Mar 2012
Yamanishi Mamoru, Kinoshita Koichiro, Fukuoka Masaki, Saito Takuya, Tanokuchi Aya, Ikeda Yuuki, Obayashi Hirokazu, Mori Koichi, Shibata Naoki, Tobimatsu Takamasa, Toraya Tetsuo
Abstract excerpt
Coenzyme B(12) dependent diol dehydratase undergoes mechanism-based inactivation by glycerol, accompanying the irreversible cleavage of the coenzyme Co-C bond. Bachovchin et al. [Biochemistry16, 1082-1092 (1977)] reported that glycerol bound in the G(S) conformation, in which the pro-S-CH(2) OH g...
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