Article
Role of tyrosine 143 in lactate dehydrogenation by flavocytochrome b2. Primary kinetic isotope effect studies with a phenylalanine mutant.
Biochemistry - 25 Jan 1994
Rouvière-Fourmy N, Capeillère-Blandin C, Lederer F
Abstract excerpt
Flavocytochrome b2 catalyzes the oxidation of lactate at the expense of cytochrome c. After flavin (FMN) reduction by the substrate, reducing equivalents are transferred one by one to heme b2, and from there on to cytochrome c. The crystal structure of the enzyme is known at 2.4-A resolution, and...
Topics
- Catalysis
- Cytochrome c Group
- Deuterium
- Flavins
- Flow Injection Analysis
- Isotope Labeling
- L-Lactate Dehydrogenase
- L-Lactate Dehydrogenase (Cytochrome)
- Lactates
- Models, Chemical
- Mutation
- Oxidation-Reduction
- Tritium
- Tyrosine