Studies of the mechanism of the delta 5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type and mutant enzymes.

doi:10.1021/bi00484a019

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Article

Studies of the mechanism of the delta 5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type and mutant enzymes.

Biochemistry - 14 Aug 1990

Xue L A, Talalay P, Mildvan A S

Abstract excerpt

delta 5-3-Ketosteroid isomerase (EC 5.3.3.1) catalyzes the isomerization of delta 5-3-ketosteroids to delta 4-3-ketosteroids by a conservative tautomeric transfer of the 4 beta-proton to the 6 beta-position using Tyr-14 as a general acid and Asp-38 as a general base [Kuliopulos, A., Mildvan, A. S...

Topics

Deuterium
Kinetics
Magnetic Resonance Spectroscopy
Mutation
Protein Conformation
Solvents
Steroid Isomerases
Substrate Specificity

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