Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.

doi:10.1073/pnas.88.6.2432

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Article

Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.

Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 1991

Persson M A, Caothien R H, Burton D R

Abstract excerpt

Combinatorial libraries of antibody heavy and light chains derived from the peripheral blood lymphocytes of an individual immunized with tetanus toxoid have been expressed in Escherichia coli by using phage lambda vectors. Screening of the libraries allowed identification of a large number of human monoclonal Fab fragments specific for tetanus toxoid. Initial studies suggested considerable sequence diversity in...

Topics

Amino Acid Sequence
Antibodies, Monoclonal
Bacteriophage lambda
Base Sequence
Cloning, Molecular
Escherichia coli
Gene Library
Genetic Variation
Genetic Vectors
Humans
Immunoglobulin Fab Fragments

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoglobulin Heavy Chains

Immunoglobulin Light Chains

Lymphocytes

Molecular Sequence Data

Oligonucleotide Probes

Plasmids

Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 1826052
DOI: 10.1073/pnas.88.6.2432
PMCID: PMC51246

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1826052-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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