By-passing immunization. Human antibodies from V-gene libraries displayed on phage.

doi:10.1016/0022-2836(91)90498-u

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Article

By-passing immunization. Human antibodies from V-gene libraries displayed on phage.

Journal of molecular biology - 5 Dec 1991

Marks J D, Hoogenboom H R, Bonnert T P, McCafferty J, Griffiths A D, Winter G

Abstract excerpt

We have mimicked features of immune selection to make human antibodies in bacteria. Diverse libraries of immunoglobulin heavy (VH) and light (V kappa and V lambda) chain variable (V) genes were prepared from peripheral blood lymphocytes (PBLs) of unimmunized donors by polymerase chain reaction (P...

Topics

Amino Acid Sequence
Bacteriophages
Base Sequence
Binding Sites, Antibody
DNA, Single-Stranded
Gene Expression Regulation, Viral
Gene Library
Humans
Immunoglobulin Fragments
Immunoglobulin Variable Region
Immunoglobulin kappa-Chains
Immunoglobulin lambda-Chains
Lymphocytes
Molecular Sequence Data
Mutation
Protein Engineering
Sequence Homology, Nucleic Acid

Source

Open in PubMed

Identifiers

PMID: 1748994
DOI: 10.1016/0022-2836(91)90498-u

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1748994-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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