Article
Real-time PCR assays for quantification and differentiation of Vibrio vulnificus strains in oysters and water.
Applied and environmental microbiology - 1 Mar 2008
Gordon Katrina V, Vickery Michael C, DePaola Angelo, Staley Christopher, Harwood Valerie J
Abstract excerpt
Vibrio vulnificus is an autochthonous estuarine bacterium and a pathogen that is frequently transmitted via raw shellfish. Septicemia can occur within 24 h; however, isolation and confirmation from water and oysters require days. Real-time PCR assays were developed to detect and differentiate two 16S rRNA variants, types A and B, which were previously associated with environmental sources and clinical fatalities,...
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