Article
A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus.
Journal of microbiological methods - 1 Feb 2007
Vickery Michael C L, Nilsson William B, Strom Mark S, Nordstrom Jessica L, DePaola Angelo
Abstract excerpt
In a terminal restriction fragment polymorphism (T-RFLP) study, we recently reported a significant association between the type B 16S rRNA gene and clinical strains of Vibrio vulnificus associated with the consumption of raw oysters. In the present study we describe a real-time PCR assay for the rapid determination of the 16S rRNA type of V. vulnificus isolates. This assay was used to reexamine the 16S rRNA gene...
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