Article

Species-specific detection of Vibrio alginolyticus in shellfish and shrimp by real-time PCR using the groEL gene

2016-02-01

Abstract excerpt

Vibrio alginolyticus is an important opportunistic pathogen in humans and marine animals. Culture-based methods and the traditional polymerase chain reaction (PCR) cannot quantify the pathogen with sufficient sensitivity. Thus, reliable, rapid, and accurate detection and quantification methods are essential to prevent and control V. alginolyticus. We developed a real-time PCR assay using SYBR Green I targeting the...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 427a9838-d4d8-5c5b-848c-983b2c0268a8
DOI: 10.1007/s10499-015-9916-5

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Species-specific detection of Vibrio alginolyticus in shellfish and shrimp by real-time PCR using the groEL geneDOI 10.1007/s10499-015-9916-5Read

Fast, simple and highly specific molecular detection of Vibrio alginolyticus pathogenic strains using a visualized isothermal amplification methodSemantic proximity Read

Real-time PCR assays for quantification and differentiation of Vibrio vulnificus strains in oysters and water.Semantic proximity Read

Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems.Semantic proximity Read

Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in ChinaSemantic proximity Read

Dual Detection of Pathogenic <em>tdh </em> and <em>trh </em>Genes of <em>Vibrio parahaemolyticus </em>in Oysters Using Multienzyme Isothermal Rapid Amplification (MIRA) Combined with Lateral-Flow Dipstick (LFD) AssaySemantic proximity Read

Improved Multiplex PCR Assay for Detection of tlh, trh, and tdh genes in Vibrio parahaemolyticus, Aligned with the U.S. FDA’s Bacteriological Analytical Manual (BAM)Semantic proximity Read

Outbreak of vibriosis caused by Vibrio harveyi and Vibrio alginolyticus in farmed seahorse Hippocampus kuda in ChinaSemantic proximity Read

Prevalence and genetic characterization of Vibrio vulnificus in raw seafood and seawater in Malaysia.Semantic proximity Read

Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular MalaysiaSemantic proximity Read

Prevalence, genetic diversity, and antimicrobial susceptibility of Vibrio spp. infected gilthead sea breams from coastal farms at Damietta, Egypt.Semantic proximity Read

Vibrio alginolyticus gyrB sequence analysis and gyrB-targeted PCR identification in environmental isolates.Semantic proximity Read

Vibrio vulnificus in aquariums is a novel threat to marine mammals and public health.Semantic proximity Read