Article
Three hTIM mutants that provide new insights on why TIM is a dimer.
Journal of molecular biology - 29 Mar 1996
Mainfroid V, Terpstra P, Beauregard M, Frère J M, Mande S C, Hol W G, Martial J A, Goraj K
Abstract excerpt
Human triosephosphate isomerase (hTIM), a dimeric enzyme, was altered by site-directed mutagenesis in order to determine whether it can be dissociated into monomers. Two hTIM mutants were produced, in which a glutamine residue was substituted for either Met14 or Arg98, both of which are interface...
Topics
- Amino Acid Sequence
- Humans
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Protein Denaturation
- Thermodynamics
- Triose-Phosphate Isomerase