Article
Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets.
Nature protocols - 1 Jan 2008
Berchowitz Luke E, Copenhaver Gregory P
Abstract excerpt
In most organisms, one crossover (CO) event inhibits the chances of another nearby event. The term used to describe this phenomenon is 'CO interference'. Here, we describe a protocol for quickly generating large data sets that are amenable to CO interference analysis in the flowering plant, Arabidopsis thaliana. We employ a visual assay that utilizes transgenic marker constructs encoding pollen-expressed...
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