Article
Involvement of the flavin si-face tyrosine on the structure and function of ferredoxin-NADP+ reductases.
The Journal of biological chemistry - 30 Nov 2001
Arakaki A K, Orellano E G, Calcaterra N B, Ottado J, Ceccarelli E A
Abstract excerpt
In ferredoxin-NADP(+) reductase (FNR), FAD is bound outside of an anti-parallel beta-barrel with the isoalloxazine lying in a two-tyrosine pocket. To elucidate the function of the flavin si-face tyrosine (Tyr-89 in pea FNR) on the enzyme structure and catalysis, we performed ab initio molecular orbital calculations and site-directed mutagenesis. Our results indicate that the position of Tyr-89 in pea FNR is...
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