Article
Rapid detection of maize DNA sequence variation.
Genetic analysis, techniques and applications - 1 Dec 1991
Shattuck-Eidens D M, Bell R N, Mitchell J T, McWhorter V C
Abstract excerpt
The allele-specific polymerase chain reaction (ASPCR) has been used to determine the genotype of maize lines at two loci, wx and NPI288. The ASPCR method uses allele-specific oligonucleotide primers in PCR amplifications to amplify and discriminate simultaneously between polymorphic alleles. The...
Topics
- Base Sequence
- Cloning, Molecular
- DNA
- Genetic Variation
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Nucleic Acid
- Zea mays