A simple and efficient non-denaturing FISH method for maize chromosome differentiation using single-strand oligonucleotide probes.

doi:10.1139/gen-2016-0167

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Article

A simple and efficient non-denaturing FISH method for maize chromosome differentiation using single-strand oligonucleotide probes.

Genome - 1 Aug 2017

Zhu Minqiu, Du Pei, Zhuang Lifang, Chu Chenggen, Zhao Han, Qi Zengjun

Abstract excerpt

Single-strand oligonucleotides (SSONs hereafter) as probes are becoming a powerful method of chromosome painting in many species. In this study, nine SSONs ((ACT)10, (ACT)19, Knob-1, Knob-2, Knob-3, CentC69-1, MR68-3, K10-72-1, and TR1-357-2) were developed and used for chromosome identification in 16 maize (Zea mays L., 2n = 20) inbred lines and hybrids by non-denaturing fluorescence in situ hybridization...

Topics

Chromosome Painting
Chromosomes, Plant
Genetic Variation
In Situ Hybridization, Fluorescence
Oligonucleotide Probes
Zea mays

Source

Open in PubMed

Identifiers

PMID: 28472606
DOI: 10.1139/gen-2016-0167

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