Article
A single nucleotide polymorphism (SNP839) in the adh1 reference gene affects the quantitation of genetically modified maize (Zea mays L.).
Journal of agricultural and food chemistry - 8 Oct 2008
Broothaerts Wim, Corbisier Philippe, Schimmel Heinz, Trapmann Stefanie, Vincent Sandra, Emons Hendrik
Abstract excerpt
The real-time PCR methods recommended in the European Union for the quantitation of genetically modified (GM) maize events NK603, GA21, and MON 863 measure the number of copies of the GM event in relation to those of the maize-specific adh1 reference gene. The study reported here revealed that the targeted 70 base pair adh1 region exhibits a single nucleotide polymorphism (SNP839) that hampers the binding of the...
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