Article

Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals

1996-11-01

Abstract excerpt

Primers for the polymerase chain reaction (PCR) were tailored to selectively amplify RFLP marker alleles associated with resistance and susceptibility for powdery mildew in cereals. The differentiation between marker alleles for susceptible and resistant genotypes is based on the discrimination of a single nucleotide by using allele-specific oligonucleotides as PCR primers. The PCR assays developed are diagnostic...

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Literature Corpus work: b00915f0-ff94-512e-a544-5ade14383176
DOI: 10.1007/s001220050338

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