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Article

Intermolecular disulfide bond formation in the NEMO dimer requires Cys54 and Cys347.

Biochemical and biophysical research communications - 29 Feb 2008

Herscovitch Melanie, Comb William, Ennis Thomas, Coleman Kate, Yong Sheila, Armstead Brinda, Kalaitzidis Demetrios, Chandani Sushil, Gilmore Thomas D

Abstract excerpt

NEMO is an essential regulatory component of the IkappaB kinase (IKK) complex, which controls activation of the NF-kappaB signaling pathway. Herein, we show that NEMO exists as a disulfide-bonded dimer when isolated from several cell types and analyzed by SDS-polyacrylamide gel electrophoresis under non-reducing conditions. Treatment of cells with hydrogen peroxide (H(2)O(2)) induces further formation of NEMO...

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Identifiers

PMID: 18164680
DOI: 10.1016/j.bbrc.2007.12.123
PMCID: PMC2277332

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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