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Article

Efficient transfection of primarily cultured porcine embryonic fibroblasts using the Amaxa Nucleofection system.

Cloning and stem cells - 1 Jan 2007

Nakayama Asuka, Sato Masahiro, Shinohara Mariko, Matsubara Shyuichiro, Yokomine Takaaki, Akasaka Eri, Yoshida Mitsutoshi, Takao Sonshin

Abstract excerpt

Porcine embryonic fibroblasts (PEF) are important as donor cells for nuclear transfer for generation of genetically modified pigs. In this study, we determined an optimal protocol for transfection of PEF with the Amaxa Nucleofection system, which directly transfers DNA into the nucleus of cells,...

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Identifiers

PMID: 18154513
DOI: 10.1089/clo.2007.0021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18154513-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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