Article
Isolation, characterization, and nuclear reprogramming of cell lines derived from porcine adult liver and fat.
Cellular reprogramming - 1 Oct 2010
Li Ping, Estrada Jose, Zhang Fan, Waghmare Sanjeev K, Mir Bashir
Abstract excerpt
Genetic manipulation of porcine genome to produce genetically modified pigs with high efficiency has been hampered by the unavailability of an ideal cell type. The cell type currently used for various genetic manipulations is fetal fibroblasts. These cells have very limited life span in culture, and efficiency of gene targeting is very low. In this study, we developed a simple but novel strategy to derive cell...
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