Article
[Application of PCR-DGGE technique in G-6-PD deficiency].
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics - 1 Dec 2007
Li Chang-Gan, Chen Xiao-Wen, Chen Yun-Sheng, Wang Ying, Zhao Wei-Ling, Shi Hong-Song, Li Cheng-Rong
Abstract excerpt
OBJECTIVE: To detect gene mutations of children with glucose-6-phosphorate dehydrogenase (G-6-PD) deficiency and of carriers of G-6-PD deficiency gene with the technique of polymerase chain reaction and denatured gradient gel electrophoresis (PCR-DGGE), and to explore the value of the technique in the diagnosis of G-6-PD deficiency and G-6-PD deficiency gene carrying. METHODS: cDNAs were harvested by reverse...
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