[A method for detecting bovine leukocyte adhesion deficiency in Holstein calves using polymerase chain reaction-single strand conformation polymorphism analysis].

Abstract excerpt

Bovine leukocyte adhesion deficiency (BLAD) is an autosomal recessive disease caused by A-->G mutation in the CD18 gene. The disease is characterized by greatly reduced expression of the heterodimeric beta2 integrin adhesion molecules on leukocytes, resulting in multiple defects in leukocyte function and significant deficits on performance traits in Holstein cattle. In this study, primers were designed to amplify...

Topics

• Animals
• Base Sequence
• Cattle
• Cattle Diseases
• Genotype
• Leukocyte-Adhesion Deficiency Syndrome
• Polymerase Chain Reaction
• Polymorphism, Single-Stranded Conformational
• Reproducibility of Results
• Sensitivity and Specificity
• Sequence Analysis, DNA
• Time Factors