Article
Technical note: Development and application of KASP assays for rapid screening of 8 genetic defects in Holstein cattle.
Journal of dairy science - 1 Jan 2020
Zhang Yi, Liang Dong, Huang Hetian, Yang Zhancheng, Wang Yachun, Yu Ying, Liu Lin, Zhang Shengli, Han Jianlin, Xiao Wei
Abstract excerpt
Specific DNA mutations underlying several genetic defects associated with embryo loss or reduced calf survivability have been identified in dairy cattle, and a convenient and cost-effective platform is required for their routine screening. We developed Kompetitive allele-specific PCR (KASP) assays for discrimination of the wild-type alleles from the associated defective alleles at each of 8 common genetic defects...
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