Article
CreA-mediated repression in Aspergillus nidulans does not require transcriptional auto-regulation, regulated intracellular localisation or degradation of CreA.
Fungal genetics and biology : FG & B - 1 May 2008
Roy Preeti, Lockington Robin A, Kelly Joan M
Abstract excerpt
The major regulatory protein in carbon repression in Aspergillus nidulans is CreA. Strains constitutively over-expressing creA show normal responses to carbon repression, indicating that auto-regulation of creA is not essential for CreA-mediated regulation. In these strains, high levels of CreA are present whether cells are grown in repressing or derepressing conditions, indicating large-scale degradation of CreA...
Topics
- Ascomycota
- Aspergillus nidulans
- Blotting, Western
- Carbon
- Cell Nucleus
- Cytoplasm
- DNA Mutational Analysis
- Fungal Proteins
- Gene Deletion
- Gene Expression Regulation, Fungal
- Genes, Reporter
- Green Fluorescent Proteins
- Microscopy, Fluorescence
- Mutation