Article
Loss of function of the carbon catabolite repressor CreA leads to low but inducer-independent expression from the feruloyl esterase B promoter in Aspergillus niger.
Biotechnology letters - 1 Jul 2021
Reijngoud Jos, Arentshorst Mark, Ruijmbeek Claudine, Reid Ian, Alazi Ebru Demirci, Punt Peter J, Tsang Adrian, Ram Arthur F J
Abstract excerpt
OBJECTIVE: With the aim to decipher the mechanisms involved in the transcriptional regulation of feruloyl esterase encoded by faeB, a genetic screen was performed to isolate A. niger mutants displaying inducer-independent expression from the faeB promoter. RESULT: PfaeB-amdS and PfaeB-lux dual reporter strains were constructed and used to isolate trans-acting mutants in which the expression of both reporters was...
Topics
- Aspergillus niger
- Carboxylic Ester Hydrolases
- Catabolite Repression
- Coumaric Acids
- Fructose
- Gene Expression Regulation, Fungal
- Genes, Reporter
- Loss of Function Mutation
- Polymorphism, Single Nucleotide