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[Expression and preliminary applications of hepatitis B surface antigen mutants].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Dec 2007

Zhu Chang-Cheng, Luo Chun-Zhen, Wang Man

Abstract excerpt

AIM: To clone and express HBsAg mutant in the Pichia pastoris. METHODS: The cloned wild type pGAP-S was used as the DNA template to generate mutant type pGAP-MS with a single or double nucleotide changes incorporated in complementary oligonucleotide primers. The product was linearized with BspH I and transformed into Pichia pastoris strain GS115, and stable multicopy integrants were screened in medium containing...

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PMID: 18062886

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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