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Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer

1 Jan 2007

Abstract excerpt

BACKGROUND: Real-time quantitative PCR (RQ-PCR) forms the basis of many breast cancer biomarker studies and novel prognostic assays, paving the way towards personalised cancer treatments. Normalisation of relative RQ-PCR data is required to control for non-biological variation introduced during sample preparation. Endogenous control (EC) genes, used in this context, should ideally be expressed constitutively and...

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PMID: 18042273
DOI: 10.1186/1471-2199-8-107

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