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Article

Measurement of the heme affinity for yeast dap1p, and its importance in cellular function.

Biochemistry - 18 Dec 2007

Thompson Alisha M, Reddi Amit R, Shi Xiaoli, Goldbeck Robert A, Moënne-Loccoz Pierre, Gibney Brian R, Holman Theodore R

Abstract excerpt

Current studies on the Saccharomyces cerevisiae protein Dap1p have demonstrated a heme-related function within the ergosterol biosynthetic pathway. Here we present data to further the understanding of the role of heme in the proper biological functioning of Dap1p in cellular processes. First, we examined the role of Dap1p in stabilizing the P450 enzyme, Erg11p, a key regulatory protein in ergosterol biosynthesis....

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Identifiers

PMID: 18031064
DOI: 10.1021/bi7013739
PMCID: PMC2669782

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