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Article

Tertiary structure in 7.9 M guanidinium chloride--the role of Glu53 and Asp287 in Pyrococcus furiosus endo-beta-1,3-glucanase.

The FEBS journal - 1 Dec 2007

Chiaraluce Roberta, Florio Rita, Angelaccio Sebastiana, Gianese Giulio, van Lieshout Johan F T, van der Oost John, Consalvi Valerio

Abstract excerpt

The thermodynamic stability of family 16 endo-beta-1,3-glucanase(EC 3.2.1.39) from the hyperthermophilic archaeon Pyrococcus furiosus is decreased upon single (D287A, E53A) and double (E53A/D287A) mutation of Asp287 and Glu53. In accordance with the homology model prediction,both carboxylic acids are involved in the composition of a calcium binding site, as shown by titration of the wild-type and the variant...

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Identifiers

PMID: 17999688
DOI: 10.1111/j.1742-4658.2007.06137.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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