Article
Osmoregulatory expression of the ompC gene in Escherichia coli K-12; IS1 insertion in the upstream regulatory region results in constitutive activation of the promoter.
FEMS microbiology letters - 15 Mar 1990
Ozawa Y, Mizushima S, Mizuno T
Abstract excerpt
A novel type of osmoregulatory mutant of Escherichia coli K-12 exhibiting constitutive expression of the ompC gene was isolated and characterized at the molecular level. In this particular mutant (cec; constitutive expression of OmpC), an insertion sequence (IS-1) was found to be located at right...
Topics
- Bacterial Outer Membrane Proteins
- Base Sequence
- DNA Transposable Elements
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genes, Regulator
- Molecular Sequence Data
- Mutation
- Porins
- Promoter Regions, Genetic
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping
- Transcription, Genetic
- Water-Electrolyte Balance