Article
Do DEAD-box proteins promote group II intron splicing without unwinding RNA?
Molecular cell - 12 Oct 2007
Del Campo Mark, Tijerina Pilar, Bhaskaran Hari, Mohr Sabine, Yang Quansheng, Jankowsky Eckhard, Russell Rick, Lambowitz Alan M
Abstract excerpt
The DEAD-box protein Mss116p promotes group II intron splicing in vivo and in vitro. Here we explore two hypotheses for how Mss116p promotes group II intron splicing: by using its RNA unwinding activity to act as an RNA chaperone or by stabilizing RNA folding intermediates. We show that an Mss116p mutant in helicase motif III (SAT/AAA), which was reported to stimulate splicing without unwinding RNA, retains...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.