Article
LeuO antagonizes H-NS and StpA-dependent repression in Salmonella enterica ompS1.
Molecular microbiology - 1 Nov 2007
De la Cruz Miguel Angel, Fernández-Mora Marcos, Guadarrama Carmen, Flores-Valdez Mario Alberto, Bustamante Víctor H, Vázquez Alejandra, Calva Edmundo
Abstract excerpt
The ompS1 gene encodes a quiescent porin in Salmonella enterica. We analysed the effects of H-NS and StpA, a paralogue of H-NS, on ompS1 expression. In an hns single mutant expression was derepressed but did not reach the maximum level. Expression in an stpA single mutant showed the same low repressed level as the wild type. In contrast, in an hns stpA background, OmpS1 became abundant in the outer membrane. The...
Topics
- Bacterial Proteins
- Base Sequence
- Blotting, Western
- DNA Footprinting
- DNA-Binding Proteins
- Deoxyribonuclease I
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation, Bacterial
- Models, Biological
- Mutation
- Porins
- Promoter Regions, Genetic
- Protein Binding