Article
Determination of allotypes G1m(f) and G1m(z) at the genomic level by subclass-specific amplification of DNA and use of allele-specific probes.
Experimental and clinical immunogenetics - 1 Jan 1991
Balbín M, Grubb A, Abrahamson M, Grubb R
Abstract excerpt
Two oligonucleotide primers were used for selective enzymatic amplification of a DNA segment encoding a major portion of the first constant region domain (CH1) of the human IgG1 heavy chain. The selective amplification was confirmed by use of subclass-specific oligonucleotide probes. Two 15-mer oligonucleotides, hybridizing with the alleles for the allotypes G1m(f) and (z), respectively, could then be used for...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- DNA
- DNA Probes
- Gene Amplification
- Genes, Immunoglobulin
- Heterozygote
- Homozygote
- Humans
- Immunoglobulin Gm Allotypes