Article
An improved method for determining high molecular weights by protein immunoblotting: application to apolipoprotein(a) phenotyping.
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society - 1 Jan 1991
Craig W Y, Forster N R, Ledue T B, Johnson A M, Neveux L M, Ritchie R F
Abstract excerpt
The determination of very high molecular weights (greater than 500 kd) by protein immunoblotting is limited by a lack of commercially available molecular weight standards in that range. We have therefore investigated the use of the human haptoglobin 2-2 phenotype polymeric series as a high molecular weight standard curve. Using pre-electrophoresed 3-12% SDS polyacrylamide gradient gels, non-dissociated...
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