Article
Molecular basis of the activity of the phytopathogen pectin methylesterase.
The EMBO journal - 5 Sept 2007
Fries Markus, Ihrig Jessica, Brocklehurst Keith, Shevchik Vladimir E, Pickersgill Richard W
Abstract excerpt
We provide a mechanism for the activity of pectin methylesterase (PME), the enzyme that catalyses the essential first step in bacterial invasion of plant tissues. The complexes formed in the crystal using specifically methylated pectins, together with kinetic measurements of directed mutants, provide clear insights at atomic resolution into the specificity and the processive action of the Erwinia chrysanthemi...
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