Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant.

doi:10.1002/yea.320070610

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Article

Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant.

Yeast (Chichester, England) - 1 Jan 2000

Butler A R, Porter M, Stark M J

Abstract excerpt

The Kluyveromyces lactis toxin is a heterotrimeric protein which irreversibly arrests proliferation of sensitive Saccharomyces cerevisiae cells in the G1 phase of the cell cycle. By expressing the gamma subunit of the toxin in sensitive yeast cells from a conditional promoter, it was previously d...

Topics

Culture Media
Galactose
Gene Expression Regulation, Fungal
Genetic Complementation Test
Interphase
Killer Factors, Yeast
Kluyveromyces
Mutation
Mycotoxins
Plasmids

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