Simultaneous screening for beta-thalassemia mutations by chemical cleavage of mismatch.

doi:10.1016/0888-7543(91)90100-s

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Article

Simultaneous screening for beta-thalassemia mutations by chemical cleavage of mismatch.

Genomics - 1 Sept 1991

Dianzani I, Camaschella C, Saglio G, Forrest S M, Ramus S, Cotton R G

Abstract excerpt

We used the chemical cleavage of mismatch (CCM) method to screen the beta-globin gene simultaneously for Mediterranean beta-thalassemia mutations. The beta-globin gene was amplified in two segments encompassing the whole gene and hybridized to a corresponding labeled PCR product from a normal sub...

Topics

Base Sequence
DNA
Genetic Techniques
Globins
Humans
Hydroxylamine
Hydroxylamines
Molecular Sequence Data
Mutation
Osmium Tetroxide
Polymerase Chain Reaction
Thalassemia

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