Article
Participation of Cys123alpha of Escherichia coli succinyl-CoA synthetase in catalysis.
Acta crystallographica. Section D, Biological crystallography - 1 Aug 2007
Hidber Esther, Brownie Edward R, Hayakawa Koto, Fraser Marie E
Abstract excerpt
Succinyl-CoA synthetase has a highly conserved cysteine residue, Cys123alpha in the Escherichia coli enzyme, that is located near the CoA-binding site and the active-site histidine residue. To test whether the succinyl moiety of succinyl-CoA is transferred to the thiol of Cys123alpha as part of the catalytic mechanism, this residue was mutated to alanine, serine, threonine and valine. Each mutant protein was...
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