Article
Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy.
FEBS letters - 24 Jul 2007
Proikas-Cezanne Tassula, Ruckerbauer Sabine, Stierhof York-Dieter, Berg Carolin, Nordheim Alfred
Abstract excerpt
Autophagy depends on the activity of phosphoinositide-3 kinase class III to generate PI(3)P. We identified the human WIPI protein family of PI(3)P-binding factors and showed that WIPI-1 (Atg18) is linked to autophagy in human cells. Induction of autophagy by rapamycin, gleevec, thapsigargin and amino acid deprivation led to an accumulation of WIPI-1 at LC3-positive membrane structures (WIPI-1 puncta-formation),...
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