Article
Simultaneous detection of Cryptosporidium parvum and Giardia in sewage sludge by IC-PCR.
Journal of applied microbiology - 1 Dec 2001
Rimhanen-Finne R, Ronkainen P, Hänninen M L
Abstract excerpt
AIMS: The aim of this study was to develop a method based on immunomagnetic capture and polymerase chain reaction (IC-PCR assay) for detection of Cryptosporidium parvum and Giardia intestinalis in sewage sludge. METHODS AND RESULTS: The detection limit of the IC-PCR assay for both organisms was 625 oocysts and cysts ml(-1). By hybridization of PCR products the sensitivity could be increased to 125 oocysts and...
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