Article
Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis.
Veterinary research communications - 1 Jan 2008
Tramuta C, Robino P, Oswald E, Nebbia P
Abstract excerpt
A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the alpha1, alpha2, beta, gamma1, gamma2/theta, kappa, epsilon, zeta, and iota intimin variants.
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.