Article
Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP.
FEMS microbiology letters - 1 Aug 2006
Lacher David W, Steinsland Hans, Whittam Thomas S
Abstract excerpt
Intimin is a highly polymorphic protein encoded by the eae gene and plays a crucial role in the attaching-effacing phenotype of diarrheagenic Escherichia coli and related pathogens. We have developed a method to quickly and accurately uncover allelic variation at the eae locus through the use of fluorescent RFLP (fRFLP). Application of fRFLP to 151 eae-positive strains (including the newly described Escherichia...
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