Article
A single silent substitution in the genome of Apple stem grooving virus causes symptom attenuation.
The Journal of general virology - 1 Sept 2003
Hirata Hisae, Lu Xiaoyun, Yamaji Yasuyuki, Kagiwada Satoshi, Ugaki Masashi, Namba Shigetou
Abstract excerpt
Among randomly mutagenized clones derived from an infectious cDNA copy of genomic RNA of Apple stem grooving virus (ASGV), we previously identified a clone, pRM21, whose in vitro transcript (ASGV-RM21) does not induce any symptoms characteristic of the original (wild-type) cDNA clone (ASGV-wt) in several host plants. Interestingly, ASGV-RM21 has only a single, translationally silent nucleotide substitution, U to...
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