Article
Analysis of thermal stress-mediated PSTVd variation and biolistic inoculation of progeny of viroid "thermomutants" to tomato and Brassica species.
Virology - 20 May 2004
Matousek Jaroslav, Orctová Lidmila, Steger Gerhard, Skopek Josef, Moors Michaela, Dedic Petr, Riesner Detlev
Abstract excerpt
Thermal stress of PSTVd-infected Nicotiana benthamiana led to appearance of a broad PSTVd sequence distribution, where most of mutations accumulated in the left half of the viroid's secondary structure including the "pathogenicity" domain. A similar effect had been reported for hop latent viroid [Virology 287 (2001) 349]. The pool of viroid "thermomutants" progenies was transcribed into cDNA and used for...
Topics
- Arabidopsis
- Base Sequence
- Biolistics
- Genetic Variation
- Heat-Shock Response
- Hot Temperature
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Plant Diseases
- Raphanus
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA