Article
The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains.
The Journal of general virology - 1 Jun 1992
Riederer M A, Grimsley N H, Hohn B, Jiricny J
Abstract excerpt
We inoculated the leaves of turnip plants (Brassica campestris spp. rapa cv. Just Right) with two cauliflower mosaic viruses (CaMVs) with different small mutations in a dispensable region of the viral genome, and followed the spread of the virus infection through the plant. Surprisingly, analysis of viral DNA in single primary chlorotic lesions revealed the presence of both mutants. In contrast, the secondary...
Topics
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA Repair
- DNA, Viral
- Gene Amplification
- Molecular Sequence Data
- Mosaic Viruses
- Mutation
- Plants
- Restriction Mapping