Article
Development of a novel nano-Invader DNA chip system.
Journal of biochemical and biophysical methods - 1 Aug 2007
Nomura Sachio, Kondo Masatoshi, Nagano Makoto, Matsui Kana, Egashira Toru
Abstract excerpt
By taking advantage of a homogeneous Invader assay, a miniaturized genotyping chip system termed nano-Invader was developed. The system is sensitive to 0.1 zeptomole of genomic DNA per well without prior PCR amplification. Its accuracy was determined by comparing both the genomic DNA chip and probe chip formats to PCR-RFLP. To determine the assay's capabilities in large-scale analysis, DNA samples from the...
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