Article
Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli.
Applied microbiology and biotechnology - 1 Oct 2007
De Muynck Cassandra, Van der Borght Jef, De Mey Marjan, De Maeseneire Sofie L, Van Bogaert Inge N A, Beauprez Joeri, Soetaert Wim, Vandamme Erick
Abstract excerpt
L-Arabinose isomerase (E.C. 5.3.1.14) catalyzes the reversible isomerization between L-arabinose and L-ribulose and is highly selective towards L-arabinose. By using a directed evolution approach, enzyme variants with altered substrate specificity were created and screened in this research. More specifically, the screening was directed towards the identification of isomerase mutants with L-ribose isomerizing...
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